sab4200841 anti tpa antibody molecular innovations  (Innovative Research Inc)

Journal: Brain and Behavior

Article Title: Jiawei Kongsheng Zhenzhong Pill (JKZP) Alleviates Chronic Cerebral Hypoperfusion‐Induced Hippocampal Synaptic Damage via S100A10/tPA/BDNF Pathway

doi: 10.1002/brb3.70328

Figure Lengend Snippet: JKZP‐containing serum may enhance the synaptic plasticity of hippocampal neurons by interfering with S100A10/tPA and modulating the proBDNF/mBDNF balance. (A) Neuronal growth process (×200); (B) MAP2 immunofluorescence to identify the purity of neurons (the purity was >95%, ×200, n = 5); (C) CCK8 screening of neurons for OGD modeling time ( n = 6); (D) CCK8 detection of the toxic effects of JKZP‐containing serum lyophilized powder on neurons ( n = 5); (E) CCK8 detection of the effects of JKZP‐containing serum lyophilized powder on neuronal activity ( n = 5). (F) RT‐qPCR assay for PSD95, GAP43, and SYN mRNA expressions ( n = 3); (G) representative images of IF staining for SYN, GAP43, and PSD95 and corresponding quantitative analyses ( n = 4, ×200); (H) WB assay for PSD95, GAP43, and SYN protein expressions ( n = 3); (I) WB assay for S100A10/tPA/BDNF pathway related protein levels ( n = 3). * p < 0.05, ** p < 0.01. JKZP, Jiawei Kongsheng Zhenzhong Pill ; mBDNF, mature BDNF; P75 NTR , P75 neurotrophic receptor; proBDNF, protein of BDNF; S100A10, S100 calcium‐binding protein A10; tPA, tissue‐type plasminogen activator; TrKB, tropomyosin receptor kinase B.

Article Snippet: Primary antibodies including growth‐associated protein 43 (GAP43, Cat. ab75810), synaptophysin (SYN, Cat. ab32127), Neun (Cat. ab177487, Abcam), MAP2 (ab254264, Abcam), and GAPDH (Cat. ab8245, Abcam) were purchased from Abcam, USA; postsynaptic density protein 95 (PSD95, Cat. 12369‐1‐AP), tPA (Cat. 10147‐1‐AP), BDNF (Cat. 28205‐1‐AP), TrkB (Cat. 13129‐1‐AP), P75 NTR (Cat. 55014‐1‐AP), sortilin (Cat. 12369‐1‐AP), and β‐actin (Cat. HRP‐60008) were purchased from Proteintech Group Inc., China; S100A10 (Cat. 201226) was purchased from Zen‐Bio, China.

Techniques: Immunofluorescence, Activity Assay, Quantitative RT-PCR, Staining, Binding Assay

Journal: Brain and Behavior

Article Title: Jiawei Kongsheng Zhenzhong Pill (JKZP) Alleviates Chronic Cerebral Hypoperfusion‐Induced Hippocampal Synaptic Damage via S100A10/tPA/BDNF Pathway

doi: 10.1002/brb3.70328

Figure Lengend Snippet: JKZP improves neuronal synaptic plasticity by regulating the balance of proBDNF/mBDNF through S100A10/tPA. (A) Fluorescence microscopy to observe the transfection rate (×200); (B) RT‐qPCR to detect the cellular S100A10 knockdown rate ( n = 4); (C and D) representative images of IF staining for SYN, GAP43, and PSD95 and corresponding quantitative analyses ( n = 4, ×200); (E) WB detection of synapse‐associated protein expression ( n = 4); (F) WB assay for S100A10/tPA/BDNF pathway related protein expression ( n = 4); (G) ELISA detection of tPA protein expression ( n = 6). * p < 0.05, ** p < 0.01. JKZP, Jiawei Kongsheng Zhenzhong Pill ; mBDNF, mature BDNF; MOI, multiplicity of infection; OGD/R, oxygen‐glucose deprivation/reperfusion; proBDNF, protein of BDNF; S100A10, S100 calcium‐binding protein A10; tPA, tissue‐type plasminogen activator.

Article Snippet: Primary antibodies including growth‐associated protein 43 (GAP43, Cat. ab75810), synaptophysin (SYN, Cat. ab32127), Neun (Cat. ab177487, Abcam), MAP2 (ab254264, Abcam), and GAPDH (Cat. ab8245, Abcam) were purchased from Abcam, USA; postsynaptic density protein 95 (PSD95, Cat. 12369‐1‐AP), tPA (Cat. 10147‐1‐AP), BDNF (Cat. 28205‐1‐AP), TrkB (Cat. 13129‐1‐AP), P75 NTR (Cat. 55014‐1‐AP), sortilin (Cat. 12369‐1‐AP), and β‐actin (Cat. HRP‐60008) were purchased from Proteintech Group Inc., China; S100A10 (Cat. 201226) was purchased from Zen‐Bio, China.

Techniques: Fluorescence, Microscopy, Transfection, Quantitative RT-PCR, Knockdown, Staining, Expressing, Enzyme-linked Immunosorbent Assay, Infection, Binding Assay

Journal: Brain and Behavior

Article Title: Jiawei Kongsheng Zhenzhong Pill (JKZP) Alleviates Chronic Cerebral Hypoperfusion‐Induced Hippocampal Synaptic Damage via S100A10/tPA/BDNF Pathway

doi: 10.1002/brb3.70328

Figure Lengend Snippet: Effects of JKZP on S100A10/tPA/BDNF pathway. (A–D) Representative images of IF staining for S100A10 and tPA, and corresponding quantitative analyses (×1000); (E) RT‐qPCR analysis of detection of mBDNF, TrkB, and P75 NTR mRNA expression; (F and G) WB analysis of BDNF pathway protein expression. * p < 0.05, ** p < 0.01. n = 3. JKZP, Jiawei Kongsheng Zhenzhong Pill ; mBDNF, mature BDNF; P75NTR, P75 neurotrophic receptor; proBDNF, protein of BDNF; S100A10, S100 calcium‐binding protein A10; tPA, tissue‐type plasminogen activator; TrKB, tropomyosin receptor kinase B.

Article Snippet: Primary antibodies including growth‐associated protein 43 (GAP43, Cat. ab75810), synaptophysin (SYN, Cat. ab32127), Neun (Cat. ab177487, Abcam), MAP2 (ab254264, Abcam), and GAPDH (Cat. ab8245, Abcam) were purchased from Abcam, USA; postsynaptic density protein 95 (PSD95, Cat. 12369‐1‐AP), tPA (Cat. 10147‐1‐AP), BDNF (Cat. 28205‐1‐AP), TrkB (Cat. 13129‐1‐AP), P75 NTR (Cat. 55014‐1‐AP), sortilin (Cat. 12369‐1‐AP), and β‐actin (Cat. HRP‐60008) were purchased from Proteintech Group Inc., China; S100A10 (Cat. 201226) was purchased from Zen‐Bio, China.

Techniques: Staining, Quantitative RT-PCR, Expressing, Binding Assay